Virulence factors and antibiotic susceptibility patterns of Klebsiella pneumonia strains Histamine producing bacteria isolated from sputum
Abdulelah A. Almayah 1 , Awatif H. Issa 2 and Hanaa k. Ibrahim 3
1 Clinical Laboratory Department, Pharmacy College, Basra University, Iraq.
2 Biology Department, Science College, Basra University, Iraq.
3 Veterinary Medicine College, Basra University, Iraq.
Objective: Histamine- producing bacteria(HPB) may be associated with much respiratory problem. Klebsiella pneumoniae is one of the most important pathogenic bacteria and is most frequently recovered from clinical specimens and can cause a classic form of primary pneumonia. Methods: Fifty-one samples of sputum collected from patients suffering from respiratory problem in the advisory clinic for chest and respiratory disease Basra city. Isolates of Klebsiella pneumoniae were identified by their morphological and biochemical characteristics. And The colony was cultured on Niven’s agar medium and. purple colony on Niven’s medium is indicator of Histamine Producing Bacteria ( HBP) according to Niven’s et al., 1981. All the isolates of Klebsiella pneumoniae identified of hdc gene by PCR and gene expression hdc detection by HPLC technique and identified were subjected to antibiotic sensitivity testing by MIC the Bio Meraux company according to16 antibiotics resistance profile and study Factors affecting Histamine production. Results: Fifty one(51) samples were collected from the patients sputum suffering from respiratory problem in the advisory clinic for chest and respiratory disease and two hundred and fifty five (255) isolates were tested using phenotypic classification based on chemical properties of bacteria. (50)strains of Klebsiella pneumoniae were identified by their morphology and biochemical characteristics. Out of above only eleven (11, 21.56%)strain of Klebsiella pneumoniae were positive for histamine production depending on the Niven´s medium as a histamine producer indicator and has been confirmed in PCR for the presence of the gene histadine decarboxylase hdc. Histamine concentration was 46.06μg/ml that produced from Klebsiella pneumoniae was detected by HPLC technology. Majority of the strains HPB isolated were sensitive to Trimethoprim . The proportion concentration of histamine produced from Klebsiella pneumoniae in pH(6.5) was 29.04μg/ml and less at a NaCl 1mg was 2.75 μg/ml. Conclusion: The K. pneumonia have a new virulence factor it can be cause or enhanced pathogenicity and high sensitive against many antibiotics Trimethoprim, while the overall resistance pattern high resistance Ampicilin, Ambicillin/Sulbactam.