Synergistic antibacterial interaction between an alum and antibiotics on some microorganism

 

Zahra Muhsin Ali 1
1 Department of Biology, College of Science, University of Kufa, Najaf, Iraq.

ABSTRACT

Objective: To determine the antibiotic resistance, antimicrobial effects of Alum and coalescence between alum and antibiotics to killing microorganisms. Methods: The tested bacteria used in this study were collected from different acute infection sources (wound, burn, axillary, and eye swab, sputum, and diarrhea) after that isolation and identification by using standard bacteriological methods. Detection antibiotic sensitivity pattern of isolates was determined by disc diffusion method, detection the antimicrobial effect of alum of isolates was determined by agar well diffusion method, and estimation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of alum also detection the combination of alum and antibiotic on isolated. Results: In this study found all isolates resistant to several antibiotics tested, all the isolates considered multi drug resistant. Antibacterial activity by inhibition zone (mm) estimation for alum on tested isolates, the inhibitory effect very observed very strong inhibition , was highest on E. coli and C. albicans ( resistance to most antibiotics) in this results observed sensitive to alum in concentration 16 % and 20% in E.coli , but in C. albicans sensitive in concentration 8%, 16%,and 20%, and other isolates sensitive to 20% alum concentration, this results showing the bacteria was highest sensitive alum than antibiotics, the minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC), all microorganisms (MOs) experiment in 20% was MIC and MBC except K. pneumoneae 20% only MIC but in C. albicans the 10% was MBC and MBC and in E. coli the 10% was MIC only. The Combination between antimicrobial agents and inhibition zone (IZ) in mm, this result know the Combination of alum with antibiotics (tetracycline and cefotaxime) highly effect than antibiotic combination, and this combination very strong in killed and destroy microorganisms and effect of alum with antibiotic the best effect than a single effect. For example, effect alum alone the IZ (38 mm) on E. coli but IZ (46 mm) combination with cefotaxime but with tetracycline IZ (40 mm), and etc. In this study, Synergistic antibacterial interaction between alum and antibiotics on all isolates. Conclusion: This study found that the antibacterial effect of Alum increases with its concentration from 8% to 20% and over concentration, the alum very high effect than antibiotics and the high effect increased with combination antibiotics. The results of agar well diffusion method similar with MIC and MBC. In summary, the showed a wide spectrum antibacterial activity. These results suggest that alum have antibacterial actions against some pathogens and may be used for the treatment and prophylaxis against diseases.