Molecular Detection of Molluscum contagiosum virus (MCV) from Patients of Basra Province / Iraq
Mohammed A. Gatea 1 , Majid N. Humoud 2 and Hayder A. Al-Hmudi 1
1 Department of Biology, College of Science, University of Basra , Basra, Iraq.
2 Department of Pathological Analysis, College of Science, University of Basra, Basra, Iraq.
ABSTRACT Objectives: To detect of Molluscum cotagiosum virus genetically using PCR technique and DNA sequencing. Methods: The present study was conducted during the period from October 2017 to April 2018 in outpatient clinic of Basrah Teaching hospital, Al-Fayha hospital, and Al-Muanaa hospital in Basrah city/Iraq. 102 samples from patients were detected and identified genetically by Polymerase chain reaction technique and confirmed by DNA sequencing. The potential MCV lesion samples of 102 patient were taken and detected genetically by PCR technique and confirmed using DNA sequencing. Results: The total number of the examined samples were 102, including 62 male and 40 female. The results showed that 79 (77.4%) patients of age group (1-11years), of which 46 (58.2%) were males and 33 (44.8%) females, 23(22.6%) of age group (twelve years or more) including 17 (73.9 %) male and 6 (26%) female. The patients age in current study ranged from 1-65 years. PCR results revealed that the size of the gene after the electrophoresis of the PCR product was 393bp and this band size also reported by other studies. Conclusion: The Molluscum contagiosum was predominantly in males and the age group 1-10 y was more infected than other age groups. Molluscum virus infections were leaded to in important histological changes in the skin, and Molecular detection of Molluscum virus was the best way to diagnose the infection.