ISSN: 2520-5234

THE EFFECT OF MUTAGENS AGENT ETHIDIUM BROMIDE ON SOME THERAPEUTIC PROPERTIES FOR LOCAL IRAQI ISOLATE Saccharomyces boulardii

Abstract

Objective: This study includes the using Ethidium bromide as as chemical mutagenic agent To Mutagenesis of local isolates Saccharomyces boulardii and tested  the changes that happen in therapeutic and Physiological Characteristics.

Methods: local isolates of  S. boulardiihas have been exposed to (5, 10, 15, 20, and 25) µg/ml of Ethidium bromide, mutants were isolated and tested against  Escherichia  coli O157:H7. Mutant Isolate gave highest antimicrobial activity was compared with wild type isolate physiology characteristics, Autoaggregation ability and cholesterol reducing.

Results: Results were as following : decreasing of survival rate by increasing of  Ethidium bromide concentration , where the survival was 11.8% at exposure to dose15µg/ml overnight,the highest percentage of mutants were induced as a result of that concentration and they gave the highest mutants 11.53%, six mutants were isolated, S. boulardii M6 culture filtrates  extract where gave the highest antimicrobial activity where diameter of  inhibition zone was 12 mm,Compared with the culture filtrates  extract of wild type S. boulardii  W.T  which was 10 mm diameter. no difference between S. boulardii  W.T  and  S. boulardii M6 in physiology characteristics where both of  isolates appeared tolerance to low pH and high concentrations of bile salts .also there was an improvement in Autoaggregation ability (significant difference P<0.05)  , it reached 69.72 % for S. boulardii M6 Compared with 65.37 % for S. boulardii  W.T. in addition to that an improvement in the ability to reduce  cholesterol level. the percentage of cholesterol reducing  after incubation 24 and 48 hrs. was 29.78%  and 57.26 % respectively, Comparison with the ability to reduce cholesterol for wild type S. boulardii W.T, which reached  25.59 and 53.90% respectively.

Conclusion: mutant local Iraqi isolate Saccharomyces boulardii by chemical agent Ethidium bromide was appeared an improvement  in antimicrobial activity against  Escherichia  coli O157:H7and in some  therapeutic characteristics Autoaggregation ability and cholesterol level lowering.

Editor in Chief
Dr.Alaa Abdul Hussein Al-Daamy

009647801273238
009647729520918

alaa.sjomr@gmail.com ; sjomr.journal@gmail.com

Editor in Chief
Dr.Alaa Abdul Hussein Al-Daamy

009647801273238
009647729520918

alaa.sjomr@gmail.com ; sjomr.journal@gmail.com

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