Isolation and Identification of Human Rhinovirus from aborted Women in Iraq

Anmar Ahmed ALTaie 1 , Basima A. Abdullah 1 and Mozahim Y. Al-Attar 2

1 Department of Microbiology, College of Science, University of Mosul, Mosul, Iraq.

2 Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.

 

ABSTRACT

Objective: The relationship between pregnant women with Bad Obstetric History (BOH) like abortion and dead fetuses and the infection with Human Rhinovirus (HRV) for the first time.

Methods: Three hundred throat swabs were collected from pregnant women with (BOH) from Mosul and Baghdad , extraced viral  RNA  from Throat swabs specimens using QIAGEN QIAamp®Viral RNA Min Kit.Germany .while Real-time PCR process for HRV was using (Fast-track FTD Respiratory pathogens 33. Luxembourg). HeLa cell line used for isolation the HRV and after the obtained the CPE and detection the HSV using TEM.

Results: Our results showed that 10% of the collected specimen were positive for the detection of (HRV) by Real Time PCR .The HRV were isolated in continuous human cell lines HeLa Cells Which detected by the appearance of characteristic cytopathic effect (CPE), which initially appears as foci of rounded up cells. The diagnosis of HRV was confirmed by using Transmission Electron Microscopy (TEM), which showed positive  appearance of HRV  as small round in shape  its size about of 30.5 nm at 200 kx.

Conclusion: There are a correlation between HRV infection with pregnant women with BOH and which diagnosed it by Real time PCR and tissue culture and determined it by TEM.